Human Adropin (AD) ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adropin (AD) .
Product Specifications
Product Name Alternative
ENHO ELISA Kit, UNQ470 ELISA Kit, Energy Homeostasis Associated Protein ELISA Kit
UniProt
Q6UWT2
Reactivity
Human
Field of Research
Signal Transduction
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Sensitivity
14.2 pg/mL
References & Citations
J Lis-Kuberka, M Berghausen-Mazur Evaluation of Selected Pro-and Anti-Inflammatory Adipokines in Colostrum from Mothers with Gestational Diabetes Mellitus International Journal of Molecular Sciences, (2024)
Notes
For research use only.
Applications Notes
Standard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human AD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human AD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AD in the samples is then determined by comparing the OD of the samples to the standard curve
Tested Applications
ELISA
Dynamic Range
31.25-2000 pg/mL
Available Sizes
Frequently Asked Questions
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