Mouse Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1) ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1) .
Product Specifications
Product Name Alternative
ALDH11 ELISA Kit, ALDH1 ELISA Kit, ALDC ELISA Kit, PUMB1 ELISA Kit, RALDH1 ELISA Kit, Retinaldehyde Dehydrogenase 1 ELISA Kit, Aldehyde dehydrogenase, cytosolic ELISA Kit, Retinal dehydrogenase 1 ELISA Kit
UniProt
P24549
Reactivity
Mouse
Field of Research
Metabolism Research
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates and other biological fluids.
Sensitivity
0.061 ng/mL
Notes
For research use only.
Applications Notes
Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ALDH1A1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ALDH1A1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ALDH1A1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ALDH1A1 in the samples is then determined by comparing the OD of the samples to the standard curve
Tested Applications
ELISA
Dynamic Range
0.16-10 ng/mL
Available Sizes
Frequently Asked Questions
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