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Mouse Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1) .

Product Specifications

Product Name Alternative

ALDH11 ELISA Kit, ALDH1 ELISA Kit, ALDC ELISA Kit, PUMB1 ELISA Kit, RALDH1 ELISA Kit, Retinaldehyde Dehydrogenase 1 ELISA Kit, Aldehyde dehydrogenase, cytosolic ELISA Kit, Retinal dehydrogenase 1 ELISA Kit

UniProt

P24549

Reactivity

Mouse

Field of Research

Metabolism Research

Assay Type

Sandwich

Assay Performance Time

3.5h

Sample Type

Serum, plasma, tissue homogenates and other biological fluids.

Sensitivity

0.061 ng/mL

Notes

For research use only.

Applications Notes

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ALDH1A1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ALDH1A1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ALDH1A1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ALDH1A1 in the samples is then determined by comparing the OD of the samples to the standard curve

Tested Applications

ELISA

Dynamic Range

0.16-10 ng/mL

Available Sizes

Frequently Asked Questions

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