Rat 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1) ELISA Kit

11DH ELISA Kit, 11-beta-HSD1 ELISA Kit, HDL ELISA Kit, HSD11 ELISA Kit, HSD11B ELISA Kit, HSD11L ELISA Kit, SDR26C1 ELISA Kit, Corticosteroid 11-beta-dehydrogenase isozyme 1 ELISA Kit, Short Chain Dehydrogenase/Reductase Family 26C, Member 1 ELISA Kit

Standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat HSD11b1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HSD11b1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat HSD11b1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat HSD11b1 in the samples is then determined by comparing the OD of the samples to the standard curve