Rat Mannose Receptor C Type 1 (MRC1) ELISA Kit

CD206 ELISA Kit, CLEC13D, CLEC13DL, MRC1L1 ELISA Kit, C-type lectin domain family 13 member D-like ELISA Kit, Macrophage mannose receptor 1-like protein 1 ELISA Kit

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MRC1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MRC1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MRC1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MRC1 in the samples is then determined by comparing the OD of the samples to the standard curve