Rat Serum Amyloid P Component (SAP) ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serum Amyloid P Component (SAP) .
Product Specifications
Product Name Alternative
APCS ELISA Kit, PTX2 ELISA Kit, Pentaxin-Related ELISA Kit, 9.5S Alpha-1-Glycoprotein ELISA Kit
UniProt
P23680
Reactivity
Rat
Field of Research
Neuroscience
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma and other biological fluids.
Sensitivity
2.51 ng/mL
Notes
For research use only.
Applications Notes
Standard: 400 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat SAP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SAP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat SAP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SAP in the samples is then determined by comparing the OD of the samples to the standard curve
Tested Applications
ELISA
Dynamic Range
6.25-400 ng/mL
Available Sizes
Frequently Asked Questions
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