Mouse Inosine Triphosphatase (ITPA) ELISA Kit

ITPase ELISA Kit, Nucleoside Triphosphate Pyrophosphatase ELISA Kit, Inosine Triphosphate Pyrophosphatase ELISA Kit, Non-canonical purine NTP pyrophosphatase ELISA Kit, Nucleoside-triphosphate diphosphatase ELISA Kit

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ITPA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ITPA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ITPA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ITPA in the samples is then determined by comparing the OD of the samples to the standard curve