Rat Heat Shock Protein Beta 8 (HSPb8) ELISA Kit

CMT2L ELISA Kit, DHMN2 ELISA Kit, E2IG1 ELISA Kit, H11 ELISA Kit, HMN2 ELISA Kit, HSP22 ELISA Kit, Heat Shock 22kDa Protein 8 ELISA Kit, Alpha-crystallin C chain ELISA Kit, E2-induced gene 1 protein ELISA Kit, Small stress protein-like protein HSP22 ELISA Kit

Standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat HSPb8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HSPb8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat HSPb8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat HSPb8 in the samples is then determined by comparing the OD of the samples to the standard curve