Rat Carbonic Anhydrase IX (CA9) ELISA Kit

CA-IX ELISA Kit, CAIX ELISA Kit, MN ELISA Kit, pMW1 ELISA Kit, P54/58N ELISA Kit, Carbonic Dehydratase ELISA Kit, Renal cell carcinoma-associated antigen G250 ELISA Kit, RCC-Associated Protein G250 ELISA Kit, Carbonate dehydratase IX ELISA Kit

Standard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CA9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CA9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CA9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CA9 in the samples is then determined by comparing the OD of the samples to the standard curve