Rat Cyclin Dependent Kinase Inhibitor 1A (CDKN1A) ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cyclin Dependent Kinase Inhibitor 1A (CDKN1A) .
Product Specifications
Product Name Alternative
WAF1 ELISA Kit, p21 ELISA Kit, CIP1 ELISA Kit, p21WAF1 ELISA Kit, CAP20 ELISA Kit, CDKN1 ELISA Kit, MDA-6 ELISA Kit, SDI1 ELISA Kit, p21CIP1 ELISA Kit, CDK-Interacting Protein 1 ELISA Kit, Melanoma differentiation-associated protein 6 ELISA Kit
Reactivity
Rat
Field of Research
Pharmacology & Drug Discovery
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Sensitivity
0.058 ng/mL
References & Citations
Reham L Younis 1, Rehab M El-Gohary 2, Asmaa A Ghalwash 2, Islam Ibrahim Hegab 1 3, Maram M Ghabrial 4, Azza M Aboshanady 4, Raghad A Mostafa 5, Alaa H Abd El-Azeem 6, Eman E Farghal 5, Asmaa A E Belal 7, Haidy Khattab Luteolin Mitigates D-Galactose-Induced Brain Ageing in Rats: SIRT1-Mediated Neuroprotection Neurochem Res, (2024)
Pubmed id
38987448
Notes
For research use only.
Applications Notes
Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CDKN1A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CDKN1A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CDKN1A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CDKN1A in the samples is then determined by comparing the OD of the samples to the standard curve
Tested Applications
ELISA
Dynamic Range
0.16-10 ng/mL
Available Sizes
Frequently Asked Questions
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