Rat Aryl Hydrocarbon Receptor Nuclear Translocator Like Protein (ARNTL) ELISA Kit

BMAL1 ELISA Kit, BMAL1c ELISA Kit, JAP3 ELISA Kit, MOP3 ELISA Kit, PASD3 ELISA Kit, TIC ELISA Kit, BHLHE5 ELISA Kit, Basic-helix-loop-helix-PAS protein MOP3 ELISA Kit, Brain and muscle ARNT-like 1 ELISA Kit, Class E basic helix-loop-helix protein 5 ELISA Kit

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ARNTL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ARNTL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ARNTL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ARNTL in the samples is then determined by comparing the OD of the samples to the standard curve