Human Interleukin 1 Family, Member 9 (IL1F9) ELISA Kit

IL-1F9 ELISA Kit, IL36g ELISA Kit, IL-1H1 ELISA Kit, IL-1RP2 ELISA Kit, IL1E ELISA Kit, IL1H1 ELISA Kit, IL1RP2 ELISA Kit, Interleukin 36, Gamma ELISA Kit, Interleukin-1 Homolog 1 ELISA Kit, Interleukin-1 epsilon ELISA Kit, Interleukin 1-Related Protein 2 ELISA Kit

Standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL1F9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL1F9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL1F9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL1F9 in the samples is then determined by comparing the OD of the samples to the standard curve