CHFR Antibody
Goat polyclonal antibody to CHFR
Product Specifications
Product Name Alternative
Anti CHFR antibody, anti checkpoint with forkhead and ring finger domains antibody, anti FLJ10796 antibody, anti FLJ33629 antibody, anti RNF116 antibody, anti RNF196 antibody, anti E3 ubiquitin-protein ligase CHFR antibody, anti RING finger protein 196 antibody
Reactivity
Human
Target
CHFR
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
KFNHICEQTRFKN
Field of Research
Epigenetics
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:64000, WB: 0.5ug/ml, IHC-P: 2.5 μg/ml
Molecular Weight
73.4; 73.3; 72.0; 69.2; 63.9
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.IHC: In paraffin embedded human brain (cerebellum) shows stained Purkinje cells. Recommended concentration, 2.5ug/ml.WB: Preliminary experiments gave an approx 80kDa band in human brain lysates at 0.5 μg/ml, this band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 72kDda according to NP_060693. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Prediction Reactivity
Bovine, Mouse, Porcine, Rat
Tested Applications
ELISA, IHC
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
55743
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