Rat Regenerating Islet Derived Protein 1 Alpha (REG1a) ELISA Kit

REG ELISA Kit, PSP ELISA Kit, ICRF ELISA Kit, PSPS1 ELISA Kit, PTP ELISA Kit, Islet cells regeneration factor ELISA Kit, Islet of Langerhans regenerating protein ELISA Kit, Lithostathine 1 Alpha ELISA Kit, Pancreatic Stone Protein ELISA Kit, Pancreatic Thread Protein ELISA Kit

Standard: 40 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat REG1a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat REG1a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat REG1a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat REG1a in the samples is then determined by comparing the OD of the samples to the standard curve