Rat Ferroportin (FPN) ELISA Kit

SLC40A1 ELISA Kit, FPN1 ELISA Kit, HFE4 ELISA Kit, IREG1 ELISA Kit, MTP1 ELISA Kit, SLC11A3 ELISA Kit, Solute Carrier Family 40, Member 1 ELISA Kit, Iron-Regulated Transporter ELISA Kit

Standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat FPN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat FPN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat FPN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat FPN in the samples is then determined by comparing the OD of the samples to the standard curve