Dog CalcitoninGene Related Peptide 1 (CGRP1) ELISA Kit

CALCA ELISA Kit, CALC1 ELISA Kit, CGRP-I ELISA Kit, CGRP1 ELISA Kit, KC ELISA Kit, CCP ELISA Kit, PDN-21 ELISA Kit, Katacalcin ELISA Kit, Calcitonin-Related Polypeptide Alpha ELISA Kit, Calcitonin carboxyl-terminal peptide ELISA Kit

Standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog CGRP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog CGRP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog CGRP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog CGRP1 in the samples is then determined by comparing the OD of the samples to the standard curve