Horse Interleukin 8 (IL8) ELISA Kit

CXCL8 ELISA Kit, AMCF-I ELISA Kit, GCP1 ELISA Kit, K60 ELISA Kit, LECT ELISA Kit, LUCT ELISA Kit, LYNAP ELISA Kit, MDNCF ELISA Kit, MONAP ELISA Kit, NAF ELISA Kit, NAP1 ELISA Kit, SCYB8 ELISA Kit, TSG1 ELISA Kit, B-ENAP ELISA Kit, Neutrophil-Activating Protein 1 ELISA Kit, Granulocyte Chemotactic Protein 1 ELISA Kit

Standard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Horse IL8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse IL8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Horse IL8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse IL8 in the samples is then determined by comparing the OD of the samples to the standard curve