Dog Stem Cell Factor (SCF) ELISA Kit

KITLG ELISA Kit, MGF ELISA Kit, SF ELISA Kit, KL1 ELISA Kit, Kitl ELISA Kit, FPH2 ELISA Kit, Kit-Ligand ELISA Kit, Steel Factor ELISA Kit, Mast Cell Growth Factor ELISA Kit, Steel factor ELISA Kit, Familial Progressive Hyperpigmentation 2 ELISA Kit

Standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog SCF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog SCF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog SCF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog SCF in the samples is then determined by comparing the OD of the samples to the standard curve