Pig Regulated on Activation in Normal T-Cell Expressed and Secreted (RANTES) ELISA Kit

CCL5 ELISA Kit, SCYA5 ELISA Kit, SISd ELISA Kit, EoCP ELISA Kit, Chemokine C-C-Motif Ligand 5 ELISA Kit, Eosinophil Chemotactic Cytokine ELISA Kit, T-cell Specific Protein p288 ELISA Kit, Small Inducible Cytokine Subfamily A (Cys-Cys) Member 5 ELISA Kit

Standard: 200 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig RANTES. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig RANTES. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig RANTES, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig RANTES in the samples is then determined by comparing the OD of the samples to the standard curve