Horse Matrix Metalloproteinase 7 (MMP7) ELISA Kit

MPSL1 ELISA Kit, MAT ELISA Kit, PUMP-1 ELISA Kit, Matrilysin Uterine ELISA Kit, Matrilysin ELISA Kit, Matrin ELISA Kit, Pump-1 Protease ELISA Kit, Uterine Metalloproteinase ELISA Kit

Standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Horse MMP7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse MMP7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Horse MMP7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse MMP7 in the samples is then determined by comparing the OD of the samples to the standard curve