Cattle Heat Shock Protein 90kDa Beta 1 (HSP90b1) ELISA Kit

HSP90-B1 ELISA Kit, ECGP ELISA Kit, GP96 ELISA Kit, GRP94 ELISA Kit, TRA1 ELISA Kit, Glycoprotein 96 ELISA Kit, Tumor Rejection Antigen (gp96) 1 ELISA Kit, 94 kDa glucose-regulated protein ELISA Kit, Endoplasmin ELISA Kit

Standard: 50 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle HSP90b1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle HSP90b1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle HSP90b1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle HSP90b1 in the samples is then determined by comparing the OD of the samples to the standard curve