Rat Annexin A2 (ANXA2) ELISA Kit

ANX-A2 ELISA Kit, ANX2L4 ELISA Kit, CAL1H ELISA Kit, LIP2 ELISA Kit, LPC2 ELISA Kit, LPC2D ELISA Kit, P36 ELISA Kit, PAP-IV ELISA Kit, Protein I ELISA Kit, Chromobindin-8 ELISA Kit, Calpactin-1 heavy chain ELISA Kit, Lipocortin II ELISA Kit, Placental anticoagulant protein IV ELISA Kit

Standard: 8000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ANXA2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ANXA2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ANXA2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ANXA2 in the samples is then determined by comparing the OD of the samples to the standard curve