Rat Neuregulin 1 (NRG1) ELISA Kit

ARIA ELISA Kit, GGF ELISA Kit, HGL ELISA Kit, HRG ELISA Kit, HRGA ELISA Kit, NDF ELISA Kit, SMDF ELISA Kit, Acetylcholine receptor-inducing activity ELISA Kit, Glial growth factor ELISA Kit, Heregulin ELISA Kit, Neu differentiation factor ELISA Kit, Sensory and motor neuron-derived factor ELISA Kit

Standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NRG1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NRG1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NRG1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NRG1 in the samples is then determined by comparing the OD of the samples to the standard curve