Pig Insulin Like Growth Factor 2 (IGF2) ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Like Growth Factor 2 (IGF2) .
Product Specifications
Product Name Alternative
INSIGF ELISA Kit, IGF-II ELISA Kit, Somatomedin A ELISA Kit, Preptin ELISA Kit, Insulin-like growth factor II Ala-25 Del ELISA Kit
UniProt
P23695
Reactivity
Porcine
Field of Research
Neuroscience; Metabolism Research; Stem Cell & Developmental Biology
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity
0.6 ng/mL
Notes
For research use only.
Applications Notes
Standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig IGF2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig IGF2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig IGF2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig IGF2 in the samples is then determined by comparing the OD of the samples to the standard curve
Tested Applications
ELISA
Dynamic Range
1.57-100 ng/mL
Available Sizes
Frequently Asked Questions
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