Rat A Disintegrin and Metalloprotease 9 (ADAM9) ELISA Kit

MCMP ELISA Kit, MDC9 ELISA Kit, Mltng ELISA Kit, Meltrin Gamma ELISA Kit, Metalloprotease/disintegrin/cysteine-rich protein 9 ELISA Kit, Myeloma cell metalloproteinase ELISA Kit, Cellular disintegrin-related protein ELISA Kit

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ADAM9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ADAM9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ADAM9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ADAM9 in the samples is then determined by comparing the OD of the samples to the standard curve