Pig Diamine Oxidase (DAO) ELISA Kit

AOC1 ELISA Kit, ABP1 ELISA Kit, KAO ELISA Kit, Histaminase ELISA Kit, Kidney amine oxidase ELISA Kit, Amiloride Binding Protein 1 ELISA Kit, Amiloride-sensitive amine oxidase ELISA Kit, Amine oxidase copper domain-containing protein 1 ELISA Kit

Standard: 200 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig DAO. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig DAO. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig DAO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig DAO in the samples is then determined by comparing the OD of the samples to the standard curve