Human Protein Arginine Methyltransferase 1 (PRMT1) ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Arginine Methyltransferase 1 (PRMT1) .
Product Specifications
Product Name Alternative
ANM1 ELISA Kit, HCP1 ELISA Kit, HRMT1L2 ELISA Kit, IR1B4 ELISA Kit, Histone-arginine N-methyltransferase PRMT1 ELISA Kit, Interferon receptor 1-bound protein 4 ELISA Kit
UniProt
Q99873
Reactivity
Human
Field of Research
Epigenetics & Chromatin
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Sensitivity
0.112 ng/mL
Notes
For research use only.
Applications Notes
Standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PRMT1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PRMT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PRMT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PRMT1 in the samples is then determined by comparing the OD of the samples to the standard curve
Tested Applications
ELISA
Dynamic Range
0.32-20 ng/mL
Available Sizes
Frequently Asked Questions
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