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Human Protein Arginine Methyltransferase 1 (PRMT1) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Arginine Methyltransferase 1 (PRMT1) .

Product Specifications

Product Name Alternative

ANM1 ELISA Kit, HCP1 ELISA Kit, HRMT1L2 ELISA Kit, IR1B4 ELISA Kit, Histone-arginine N-methyltransferase PRMT1 ELISA Kit, Interferon receptor 1-bound protein 4 ELISA Kit

UniProt

Q99873

Reactivity

Human

Field of Research

Epigenetics & Chromatin

Assay Type

Sandwich

Assay Performance Time

3.5h

Sample Type

Tissue homogenates, cell lysates and other biological fluids

Sensitivity

0.112 ng/mL

Notes

For research use only.

Applications Notes

Standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PRMT1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PRMT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PRMT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PRMT1 in the samples is then determined by comparing the OD of the samples to the standard curve

Tested Applications

ELISA

Dynamic Range

0.32-20 ng/mL

Available Sizes

Frequently Asked Questions

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