Human Chemokine C-C-Motif Ligand 16 (CCL16) ELISA Kit

SCYA16 ELISA Kit, SCYL4 ELISA Kit, LEC ELISA Kit, HCC4 ELISA Kit, LMC ELISA Kit, LCC1 ELISA Kit, CKb12 ELISA Kit, Mtn1 ELISA Kit, CC-4 ELISA Kit, Monotactin-1 ELISA Kit, IL-10-inducible chemokine ELISA Kit, Liver-expressed chemokine ELISA Kit, Lymphocyte and monocyte chemoattractant ELISA Kit

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CCL16. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CCL16. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CCL16, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CCL16 in the samples is then determined by comparing the OD of the samples to the standard curve