Rabbit anti Porcine Elastase
Product Specifications
Background
The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition. This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques); to prepare an insoluble immuno-affinity adsorbent; for labelling with a marker of the customer’s own choice.
Host
Rabbit
Clone
Polyclonal
Source
Elastase isolated and purified from porcine pancreas. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Assay Principle
Immunoprecipitation.
Form
Purified hyperimmune rabbit IgG antibodies lyophilised from a solution in phosphate buffered saline (PBS, pH 7,2), stabilized with dextran; No preservative added, as it may interfere with the antibody activity; No foreign protein added; It is reconstituted by adding 0,5 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C
Precautions
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
Storage Conditions
The lyophilised PAb fraction is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C; Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2); Repeated thawing and freezing should be avoided; Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day; If a slight precipitation occurs upon storage, this should be removed by centrifugation; It will not affect the performance of the product
CAS Number
9007-83-4
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