Human Protein Phosphatase, Mg2+/Mn2+ Dependent 1B (PPM1B) ELISA Kit

PP2C-beta-X ELISA Kit, PP2CB ELISA Kit, PP2CBETA ELISA Kit, PPC2BETAX ELISA Kit, Protein Phosphatase 2C, Beta Isoform ELISA Kit, Protein Phosphatase 1B (formerly 2C), Magnesium-Dependent, Beta Isoform ELISA Kit

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PPM1B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PPM1B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PPM1B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PPM1B in the samples is then determined by comparing the OD of the samples to the standard curve