Human Mothers Against Decapentaplegic Homolog 1 (Smad1) ELISA Kit

BSP1 ELISA Kit, JV4-1 ELISA Kit, JV41 ELISA Kit, MADH1 ELISA Kit, MADR1 ELISA Kit, SMAD Family Member 1 ELISA Kit, Mad-related protein 1 ELISA Kit, Transforming growth factor-beta-signaling protein 1 ELISA Kit, Mothers against DPP homolog 1 ELISA Kit

Standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Smad1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Smad1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Smad1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Smad1 in the samples is then determined by comparing the OD of the samples to the standard curve