Rat Mucin 5 Subtype B (MUC5B) ELISA Kit

MUC5-B ELISA Kit, MUC9 ELISA Kit, MG1 ELISA Kit, Oligomeric Mucus/Gel-Forming ELISA Kit, Mucin 5, Subtype B, Tracheobronchial ELISA Kit, Cervical mucin ELISA Kit, High molecular weight salivary mucin MG1 ELISA Kit, Sublingual gland mucin ELISA Kit

Standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MUC5B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MUC5B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MUC5B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MUC5B in the samples is then determined by comparing the OD of the samples to the standard curve