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Human Adaptor Related Protein Complex 3 Beta 1 (AP3b1) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adaptor Related Protein Complex 3 Beta 1 (AP3b1) .

Product Specifications

Product Name Alternative

HPS ELISA Kit, PE ELISA Kit, HPS2 ELISA Kit, ADTB3 ELISA Kit, ADTB3A ELISA Kit, Clathrin assembly protein complex 3 beta-1 large chain ELISA Kit, Adaptor protein complex AP-3 subunit beta-1 ELISA Kit

UniProt

O00203

Reactivity

Human

Field of Research

Signal Transduction

Assay Type

Sandwich

Assay Performance Time

3.5h

Sample Type

Tissue homogenates, cell lysates and other biological fluids

Sensitivity

0.065 ng/mL

Notes

For research use only.

Applications Notes

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human AP3b1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AP3b1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human AP3b1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AP3b1 in the samples is then determined by comparing the OD of the samples to the standard curve

Tested Applications

ELISA

Dynamic Range

0.16-10 ng/mL

Available Sizes

Frequently Asked Questions

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