Rat Thyroid Hormone Receptor Beta (THRb) ELISA Kit

ERBA-BETA ELISA Kit, ERBA2 ELISA Kit, GRTH ELISA Kit, NR1A2 ELISA Kit, THR1 ELISA Kit, THRB1 ELISA Kit, THRB2 ELISA Kit, Erythroblastic Leukemia Viral (v-erb-a) Oncogene Homolog 2, Avian ELISA Kit, Nuclear Receptor Subfamily 1, Group A, Member 2 ELISA Kit

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat THRb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat THRb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat THRb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat THRb in the samples is then determined by comparing the OD of the samples to the standard curve