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PAX8 (N Terminus) Antibody

Goat polyclonal antibody to PAX8

Product Specifications

Product Name Alternative

Anti PAX8 antibody, anti paired box gene 8 antibody, anti paired domain gene 8 antibody, anti paired box 8 antibody

Reactivity

Human

Target

PAX8 (N Terminus)

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

PHNSIRSGHGGLN

Field of Research

Stem Cell & Developmental Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:128000, WB: 1-2 μg/ml, IHC-P: 5 μg/ml

Molecular Weight

48.2; 41.7; 43.0; 34.9; 31.0

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Thyroid Gland shows nuclear staining in epithelial cells around follicles. Recommended concentration, 1-2ug/ml.A customer found this antibody unsuccessful in immunohistochemistry on Mouse tissues.WB: Approx 40kDa band observed in Human Liver lysates (calculated MW of xxkDa according to NP_039245.1) . Recommended concentration: 1-3 μg/ml.

Prediction Reactivity

Mouse, Rat

Tested Applications

ELISA, IHC, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

7849

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