Rat Glutathione S Transferase Mu 1 (GSTm1) ELISA Kit

GST-M1 ELISA Kit, GST1 ELISA Kit, GSTM1-1 ELISA Kit, GSTM1a-1a ELISA Kit, GSTM1b-1b ELISA Kit, GTH4 ELISA Kit, GTM1 ELISA Kit, H-B ELISA Kit, MU1 ELISA Kit, GST HB subunit 4 ELISA Kit

Standard: 400 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat GSTm1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GSTm1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat GSTm1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat GSTm1 in the samples is then determined by comparing the OD of the samples to the standard curve