Human Homing Associated Cell Adhesion Molecule (HCAM) ELISA Kit

CDW44 ELISA Kit, CD44 ELISA Kit, CSPG8 ELISA Kit, ECMR-III ELISA Kit, HCELL ELISA Kit, IN ELISA Kit, LHR ELISA Kit, MC56 ELISA Kit, MDU2 ELISA Kit, MDU3 ELISA Kit, MIC4 ELISA Kit, MUTCH-I ELISA Kit, Pgp1 ELISA Kit, Hematopoietic Cell E- And L-Selectin Ligand ELISA Kit, Chondroitin Sulfate Proteoglycan 8 ELISA Kit

Standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HCAM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HCAM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HCAM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HCAM in the samples is then determined by comparing the OD of the samples to the standard curve