Human Mannose-6-Phosphate Receptor (M6PR) ELISA Kit

SMPR ELISA Kit, MPR46 ELISA Kit, MPRD ELISA Kit, Cation Dependent Mannose 6-Phosphate Receptor ELISA Kit, 46 kDa mannose 6-phosphate receptor ELISA Kit

Standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human M6PR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human M6PR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human M6PR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human M6PR in the samples is then determined by comparing the OD of the samples to the standard curve