Rat Phospholipase A2, Calcium Independent (iPLA2) ELISA Kit

PLA2G6 ELISA Kit, CaI-PLA2 ELISA Kit, GVI ELISA Kit, INAD1 ELISA Kit, PNPLA9 ELISA Kit, Ca2+ Independent PLA2 ELISA Kit, 85 kDa Calcium-Independent Phospholipase A2 ELISA Kit, Phospholipase A2, group VI (Cytosolic, Calcium-Independent) ELISA Kit

Standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat iPLA2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat iPLA2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat iPLA2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat iPLA2 in the samples is then determined by comparing the OD of the samples to the standard curve