Human Huntingtin Interacting Protein 2 (HIP2) ELISA Kit

HYPG ELISA Kit, LIG ELISA Kit, UBE2K ELISA Kit, Ubiquitin-Conjugating Enzyme E2K ELISA Kit, Ubiquitin carrier protein ELISA Kit, Ubiquitin-protein ligase ELISA Kit

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HIP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HIP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HIP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HIP2 in the samples is then determined by comparing the OD of the samples to the standard curve