Mouse Immune Receptor Expressed on Myeloid Cells 1 (IREM1) ELISA Kit

CD300f ELISA Kit, CD300LF ELISA Kit, NKIR ELISA Kit, IGSF13 ELISA Kit, CLM1 ELISA Kit, CD300 Antigen Like Family Member F ELISA Kit, CMRF35-Like Molecule 1 ELISA Kit, Immunoglobulin superfamily member 13 ELISA Kit, NK inhibitory receptor ELISA Kit

Standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IREM1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IREM1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IREM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IREM1 in the samples is then determined by comparing the OD of the samples to the standard curve