Rat Extracellular Signal Regulated Kinase 2 (ERK2) ELISA Kit

MAPK1 ELISA Kit, P38 ELISA Kit, P40 ELISA Kit, ERK2 ELISA Kit, ERT1 ELISA Kit, MAPK2 ELISA Kit, P42MAPK ELISA Kit, P42-MAPK ELISA Kit, PRKM1 ELISA Kit, PRKM2 ELISA Kit, P41 ELISA Kit, P41mapk ELISA Kit, P41-mapk ELISA Kit, Mitogen-Activated Protein Kinase 1 ELISA Kit, Mitogen-activated protein kinase 2 ELISA Kit

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ERK2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ERK2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ERK2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ERK2 in the samples is then determined by comparing the OD of the samples to the standard curve