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Human Creatine Kinase, Mitochondrial 2, Sarcomeric (CKMT2) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Creatine Kinase, Mitochondrial 2, Sarcomeric (CKMT2) .

Product Specifications

Product Name Alternative

MtCK ELISA Kit, CK-MT2 ELISA Kit, SMTCK ELISA Kit, Mib-CK ELISA Kit, Creatine Kinase S-type ELISA Kit, Basic-type mitochondrial creatine kinase ELISA Kit, Sarcomeric mitochondrial creatine kinase ELISA Kit

UniProt

P17540

Reactivity

Human

Field of Research

Pharmacology & Drug Discovery

Assay Type

Sandwich

Assay Performance Time

3.5h

Sample Type

Tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Sensitivity

0.6 ng/mL

Notes

For research use only.

Applications Notes

Standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CKMT2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CKMT2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CKMT2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CKMT2 in the samples is then determined by comparing the OD of the samples to the standard curve

Tested Applications

ELISA

Dynamic Range

1.57-100 ng/mL

Available Sizes

Frequently Asked Questions

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