Human 17-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD17b1) ELISA Kit

EDH17B2 ELISA Kit, EDHB17 ELISA Kit, HSD17 ELISA Kit, SDR28C1 ELISA Kit, Estradiol 17-Beta-Dehydrogenase 1 ELISA Kit, Short Chain Dehydrogenase/Reductase Family 28CE,1 ELISA Kit, 20 alpha-hydroxysteroid dehydrogenase ELISA Kit

Standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HSD17b1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HSD17b1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HSD17b1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HSD17b1 in the samples is then determined by comparing the OD of the samples to the standard curve