Rat Protein Zero, Myelin (MPZ) ELISA Kit

P0 ELISA Kit, DSS ELISA Kit, CHM ELISA Kit, CMT4E ELISA Kit, CMT1 ELISA Kit, CMT1B ELISA Kit, CMT2I ELISA Kit, CMT2J ELISA Kit, CMTDI3 ELISA Kit, HMSNIB ELISA Kit, MPP ELISA Kit, Myelin Protein Zero ELISA Kit, Charcot-Marie-Tooth Neuropathy 1B ELISA Kit, Myelin peripheral protein ELISA Kit

Standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MPZ. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MPZ. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MPZ, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MPZ in the samples is then determined by comparing the OD of the samples to the standard curve