Human S-Phase Kinase Associated Protein 1 (SKP1) ELISA Kit

SKP1A ELISA Kit, EMC19 ELISA Kit, OCP-II ELISA Kit, OCP2 ELISA Kit, TCEB1L ELISA Kit, p19A ELISA Kit, Cyclin-A/CDK2-associated p19 ELISA Kit, RNA polymerase II elongation factor-like protein ELISA Kit, Transcription elongation factor B polypeptide 1-like ELISA Kit

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SKP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SKP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SKP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SKP1 in the samples is then determined by comparing the OD of the samples to the standard curve