Human Na+ Taurocholate Cotransporting Polypeptide (NTCP) ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Na+ Taurocholate Cotransporting Polypeptide (NTCP) .
Product Specifications
Product Name Alternative
SLC10A1 ELISA Kit, NTCP1 ELISA Kit, Solute Carrier Family 10 Member 1, Sodium/Bile Acid Cotransporter Family ELISA Kit, Cell growth-inhibiting gene 29 protein ELISA Kit, Na (+) /bile acid cotransporter ELISA Kit
Reactivity
Human
Field of Research
Signal Transduction
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Sensitivity
0.28 ng/mL
Notes
For research use only.
Applications Notes
Standard: 50 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NTCP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NTCP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NTCP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NTCP in the samples is then determined by comparing the OD of the samples to the standard curve
Tested Applications
ELISA
Dynamic Range
0.79-50 ng/mL
Available Sizes
Frequently Asked Questions
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