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Human Na+ Taurocholate Cotransporting Polypeptide (NTCP) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Na+ Taurocholate Cotransporting Polypeptide (NTCP) .

Product Specifications

Product Name Alternative

SLC10A1 ELISA Kit, NTCP1 ELISA Kit, Solute Carrier Family 10 Member 1, Sodium/Bile Acid Cotransporter Family ELISA Kit, Cell growth-inhibiting gene 29 protein ELISA Kit, Na (+) /bile acid cotransporter ELISA Kit

Reactivity

Human

Field of Research

Signal Transduction

Assay Type

Sandwich

Assay Performance Time

3.5h

Sample Type

Tissue homogenates, cell lysates and other biological fluids

Sensitivity

0.28 ng/mL

Notes

For research use only.

Applications Notes

Standard: 50 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NTCP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NTCP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NTCP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NTCP in the samples is then determined by comparing the OD of the samples to the standard curve

Tested Applications

ELISA

Dynamic Range

0.79-50 ng/mL

Available Sizes

Frequently Asked Questions

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