Human ATP Binding Cassette Transporter C4 (ABCC4) ELISA Kit

ABC-C4 ELISA Kit, MRP4 ELISA Kit, MOATB ELISA Kit, Canalicular Multispecific Organic Anion Transporter B ELISA Kit, Multidrug Resistance-Associated Protein 4 ELISA Kit, Multi-specific organic anion transporter B ELISA Kit

Standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ABCC4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ABCC4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ABCC4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ABCC4 in the samples is then determined by comparing the OD of the samples to the standard curve