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Human Cyclin Dependent Kinase 16 (CDK16) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cyclin Dependent Kinase 16 (CDK16) .

Product Specifications

Product Name Alternative

PCTK1 ELISA Kit, PCTAIRE ELISA Kit, PCTAIRE1 ELISA Kit, PCTGAIRE ELISA Kit, Serine/Threonine-Protein Kinase PCTAIRE-1 ELISA Kit, PCTAIRE-motif protein kinase 1 ELISA Kit, Cell division protein kinase 16 ELISA Kit

UniProt

Q00536

Reactivity

Human

Field of Research

Signal Transduction

Assay Type

Sandwich

Assay Performance Time

3.5h

Sample Type

Tissue homogenates, cell lysates and other biological fluids.

Sensitivity

12.6 pg/mL

Notes

For research use only.

Applications Notes

Standard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CDK16. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CDK16. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CDK16, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CDK16 in the samples is then determined by comparing the OD of the samples to the standard curve

Tested Applications

ELISA

Dynamic Range

31.25-2000 pg/mL

Available Sizes

Frequently Asked Questions

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