Human Frizzled Related Protein (FRZB) ELISA Kit

FRZB-PEN ELISA Kit, FRZB1 ELISA Kit, SRFP3 ELISA Kit, FRP-3 ELISA Kit, SFRP3 ELISA Kit, FRE ELISA Kit, FRITZ ELISA Kit, FRZB-1 ELISA Kit, FZRB ELISA Kit, hFIZ ELISA Kit, Secreted frizzled-related protein 3 ELISA Kit

Standard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FRZB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FRZB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FRZB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FRZB in the samples is then determined by comparing the OD of the samples to the standard curve