Human Aurora Kinase B (AURKB) ELISA Kit

AIM1 ELISA Kit, AIK2 ELISA Kit, ARK2 ELISA Kit, AurB ELISA Kit, IPL1 ELISA Kit, STK1 ELISA Kit, STK12 ELISA Kit, STK5 ELISA Kit, Serine/Threonine Kinase 12 ELISA Kit, Aurora-1 ELISA Kit, Aurora-and IPL1-like midbody-associated protein 1 ELISA Kit, Aurora/IPL1-related kinase 2 ELISA Kit

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human AURKB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AURKB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human AURKB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AURKB in the samples is then determined by comparing the OD of the samples to the standard curve